Construct: shRNA TRCN0000419743

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003786.3-3699s21c1
DNA Barcode:
GTGCTCTTTGCTGCACTATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ABCC3 (8714)
Gene Description:
ATP binding cassette subfamily C member 3
Transcript:
RefSeq NM_003786.3 (NON-CURRENT)
Match location:
Position 3699 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000419743)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGCTCTTTGCTGCACTATTT
Hairpin Sequence:
5'-CCGG-GTGCTCTTTGCTGCACTATTT-CTCGAG-AAATAGTGCAGCAAAGAGCAC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGCTCTTTGCTGCACTATTTCTCGAGAAATAGTGCAGCAAAGAGCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGCTCTTTGCTGCACTATTTCTCGAGAAATAGTGCAGCAAAGAGCAC-3'

Other clones with same target sequence:

(none)