Construct: shRNA TRCN0000419761

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_138569.2-1544s21c1
DNA Barcode:
GCTAACCACTTGCTAGATTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MLIP (90523)
Gene Description:
muscular LMNA interacting protein
Transcript:
RefSeq NM_138569.2 (CURRENT)
Match location:
Position 1544 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000419761)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTAACCACTTGCTAGATTTA
Hairpin Sequence:
5'-CCGG-GCTAACCACTTGCTAGATTTA-CTCGAG-TAAATCTAGCAAGTGGTTAGC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTAACCACTTGCTAGATTTACTCGAGTAAATCTAGCAAGTGGTTAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTAACCACTTGCTAGATTTACTCGAGTAAATCTAGCAAGTGGTTAGC-3'

Other clones with same target sequence:

(none)