Construct: shRNA TRCN0000419801

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020453.3-4813s21c1
DNA Barcode:
GTCAGGCTCCAGTCGGAATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ATP10D (57205)
Gene Description:
ATPase phospholipid transporting 10D (putative)
Transcript:
RefSeq NM_020453.3 (NON-CURRENT)
Match location:
Position 4813 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000419801)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCAGGCTCCAGTCGGAATTT
Hairpin Sequence:
5'-CCGG-GTCAGGCTCCAGTCGGAATTT-CTCGAG-AAATTCCGACTGGAGCCTGAC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCAGGCTCCAGTCGGAATTTCTCGAGAAATTCCGACTGGAGCCTGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCAGGCTCCAGTCGGAATTTCTCGAGAAATTCCGACTGGAGCCTGAC-3'

Other clones with same target sequence:

(none)