Construct: shRNA TRCN0000419965

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153356.1-268s21c1
DNA Barcode:
TGAGGACTGAAGCCTGGAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TBC1D21 (161514)
Gene Description:
TBC1 domain family member 21
Transcript:
RefSeq NM_153356.1 (NON-CURRENT)
Match location:
Position 268 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000419965)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGAGGACTGAAGCCTGGAAAT
Hairpin Sequence:
5'-CCGG-TGAGGACTGAAGCCTGGAAAT-CTCGAG-ATTTCCAGGCTTCAGTCCTCA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGAGGACTGAAGCCTGGAAATCTCGAGATTTCCAGGCTTCAGTCCTCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGAGGACTGAAGCCTGGAAATCTCGAGATTTCCAGGCTTCAGTCCTCA-3'

Other clones with same target sequence:

(none)