Construct: shRNA TRCN0000420000

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023068.3-2222s21c1
DNA Barcode:
ACTTCTCCTGGTTCCGAAATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SIGLEC1 (6614)
Gene Description:
sialic acid binding Ig like lectin 1
Transcript:
RefSeq NM_023068.3 (NON-CURRENT)
Match location:
Position 2222 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000420000)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACTTCTCCTGGTTCCGAAATG
Hairpin Sequence:
5'-CCGG-ACTTCTCCTGGTTCCGAAATG-CTCGAG-CATTTCGGAACCAGGAGAAGT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACTTCTCCTGGTTCCGAAATGCTCGAGCATTTCGGAACCAGGAGAAGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACTTCTCCTGGTTCCGAAATGCTCGAGCATTTCGGAACCAGGAGAAGT-3'

Other clones with same target sequence:

(none)