Construct: shRNA TRCN0000420060

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144709.2-536s21c1
DNA Barcode:
GCTTCCAAGAACTCAAATTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PUS10 (150962)
Gene Description:
pseudouridine synthase 10
Transcript:
RefSeq NM_144709.2 (NON-CURRENT)
Match location:
Position 536 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000420060)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTCCAAGAACTCAAATTTA
Hairpin Sequence:
5'-CCGG-GCTTCCAAGAACTCAAATTTA-CTCGAG-TAAATTTGAGTTCTTGGAAGC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTCCAAGAACTCAAATTTACTCGAGTAAATTTGAGTTCTTGGAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTCCAAGAACTCAAATTTACTCGAGTAAATTTGAGTTCTTGGAAGC-3'

Other clones with same target sequence:

(none)