Construct: shRNA TRCN0000420063

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000829.3-3136s21c1
DNA Barcode:
TTGCATCGGACCTACCATAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GRIA4 (2893)
Gene Description:
glutamate ionotropic receptor AMPA type subunit 4
Transcript:
RefSeq NM_000829.3 (NON-CURRENT)
Match location:
Position 3136 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000420063)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTGCATCGGACCTACCATAAA
Hairpin Sequence:
5'-CCGG-TTGCATCGGACCTACCATAAA-CTCGAG-TTTATGGTAGGTCCGATGCAA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTGCATCGGACCTACCATAAACTCGAGTTTATGGTAGGTCCGATGCAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTGCATCGGACCTACCATAAACTCGAGTTTATGGTAGGTCCGATGCAA-3'

Other clones with same target sequence:

(none)