Construct: shRNA TRCN0000420069

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006739.3-263s21c1
DNA Barcode:
GATGAACTCAAGCGGCATTAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MCM5 (4174)
Gene Description:
minichromosome maintenance complex component 5
Transcript:
RefSeq NM_006739.3 (NON-CURRENT)
Match location:
Position 263 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000420069)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATGAACTCAAGCGGCATTAC
Hairpin Sequence:
5'-CCGG-GATGAACTCAAGCGGCATTAC-CTCGAG-GTAATGCCGCTTGAGTTCATC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATGAACTCAAGCGGCATTACCTCGAGGTAATGCCGCTTGAGTTCATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATGAACTCAAGCGGCATTACCTCGAGGTAATGCCGCTTGAGTTCATC-3'

Other clones with same target sequence:

(none)