Construct: shRNA TRCN0000420140

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001010880.2-1596s21c1
DNA Barcode:
TTGCCAACTTATTGAACATTC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZNF780A (284323)
Gene Description:
zinc finger protein 780A
Transcript:
RefSeq NM_001010880.2 (CURRENT)
Match location:
Position 1596 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000420140)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTGCCAACTTATTGAACATTC
Hairpin Sequence:
5'-CCGG-TTGCCAACTTATTGAACATTC-CTCGAG-GAATGTTCAATAAGTTGGCAA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTGCCAACTTATTGAACATTCCTCGAGGAATGTTCAATAAGTTGGCAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTGCCAACTTATTGAACATTCCTCGAGGAATGTTCAATAAGTTGGCAA-3'

Other clones with same target sequence:

(none)