Construct: shRNA TRCN0000420148

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013357.2-1504s21c1
DNA Barcode:
GAAATTGACCTCCGGTATATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PURG (29942)
Gene Description:
purine rich element binding protein G
Transcript:
RefSeq NM_013357.2 (CURRENT)
Match location:
Position 1504 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000420148)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAATTGACCTCCGGTATATA
Hairpin Sequence:
5'-CCGG-GAAATTGACCTCCGGTATATA-CTCGAG-TATATACCGGAGGTCAATTTC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAATTGACCTCCGGTATATACTCGAGTATATACCGGAGGTCAATTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAATTGACCTCCGGTATATACTCGAGTATATACCGGAGGTCAATTTC-3'

Other clones with same target sequence:

(none)