Construct: shRNA TRCN0000420175

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_183172.1-1923s21c1
DNA Barcode:
CATTAACTGGTTTACTCATTG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ric8b (237422)
Gene Description:
RIC8 guanine nucleotide exchange factor B
Transcript:
RefSeq NM_183172.1 (NON-CURRENT)
Match location:
Position 1923 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000420175)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATTAACTGGTTTACTCATTG
Hairpin Sequence:
5'-CCGG-CATTAACTGGTTTACTCATTG-CTCGAG-CAATGAGTAAACCAGTTAATG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATTAACTGGTTTACTCATTGCTCGAGCAATGAGTAAACCAGTTAATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATTAACTGGTTTACTCATTGCTCGAGCAATGAGTAAACCAGTTAATG-3'

Other clones with same target sequence:

(none)