Construct: shRNA TRCN0000420258

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144645.3-667s21c1
DNA Barcode:
ACACCTGAAGCGCGGATTGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
C4orf36 (132989)
Gene Description:
chromosome 4 open reading frame 36
Transcript:
RefSeq NM_144645.3 (NON-CURRENT)
Match location:
Position 667 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000420258)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACACCTGAAGCGCGGATTGTA
Hairpin Sequence:
5'-CCGG-ACACCTGAAGCGCGGATTGTA-CTCGAG-TACAATCCGCGCTTCAGGTGT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACACCTGAAGCGCGGATTGTACTCGAGTACAATCCGCGCTTCAGGTGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACACCTGAAGCGCGGATTGTACTCGAGTACAATCCGCGCTTCAGGTGT-3'

Other clones with same target sequence:

(none)