Construct: shRNA TRCN0000420275

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_203406.1-1050s21c1
DNA Barcode:
TATGATGGATCACTGATTGAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MBLAC2 (153364)
Gene Description:
metallo-beta-lactamase domain containing 2
Transcript:
RefSeq NM_203406.1 (NON-CURRENT)
Match location:
Position 1050 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000420275)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TATGATGGATCACTGATTGAC
Hairpin Sequence:
5'-CCGG-TATGATGGATCACTGATTGAC-CTCGAG-GTCAATCAGTGATCCATCATA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTATGATGGATCACTGATTGACCTCGAGGTCAATCAGTGATCCATCATATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATATGATGGATCACTGATTGACCTCGAGGTCAATCAGTGATCCATCATA-3'

Other clones with same target sequence:

(none)