Construct: shRNA TRCN0000420311

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145287.2-900s21c1
DNA Barcode:
ATCACATCTAAAGGGACATAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZNF519 (162655)
Gene Description:
zinc finger protein 519
Transcript:
RefSeq NM_145287.2 (NON-CURRENT)
Match location:
Position 900 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000420311)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATCACATCTAAAGGGACATAA
Hairpin Sequence:
5'-CCGG-ATCACATCTAAAGGGACATAA-CTCGAG-TTATGTCCCTTTAGATGTGAT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATCACATCTAAAGGGACATAACTCGAGTTATGTCCCTTTAGATGTGATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATCACATCTAAAGGGACATAACTCGAGTTATGTCCCTTTAGATGTGAT-3'

Other clones with same target sequence:

(none)