Construct: shRNA TRCN0000420328

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028177.3-492s21c1
DNA Barcode:
GAATAAAGTATCAGATGTCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ndufab1 (70316)
Gene Description:
NADH dehydrogenase (ubiquinone) 1, alpha/beta subcomplex, 1
Transcript:
RefSeq NM_028177.3 (CURRENT)
Match location:
Position 492 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000420328)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAATAAAGTATCAGATGTCTT
Hairpin Sequence:
5'-CCGG-GAATAAAGTATCAGATGTCTT-CTCGAG-AAGACATCTGATACTTTATTC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAATAAAGTATCAGATGTCTTCTCGAGAAGACATCTGATACTTTATTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAATAAAGTATCAGATGTCTTCTCGAGAAGACATCTGATACTTTATTC-3'

Other clones with same target sequence:

(none)