Construct: shRNA TRCN0000420393

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002541.3-3607s21c1
DNA Barcode:
AGGCACAGGCTCCTGTATTTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
OGDH (4967)
Gene Description:
oxoglutarate dehydrogenase
Transcript:
RefSeq NM_002541.3 (NON-CURRENT)
Match location:
Position 3607 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000420393)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGGCACAGGCTCCTGTATTTG
Hairpin Sequence:
5'-CCGG-AGGCACAGGCTCCTGTATTTG-CTCGAG-CAAATACAGGAGCCTGTGCCT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGGCACAGGCTCCTGTATTTGCTCGAGCAAATACAGGAGCCTGTGCCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGGCACAGGCTCCTGTATTTGCTCGAGCAAATACAGGAGCCTGTGCCT-3'

Other clones with same target sequence:

(none)