Construct: shRNA TRCN0000420399

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177749.3-674s21c1
DNA Barcode:
CATCAAGATCACAGGTATATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Kir3dl1 (245616)
Gene Description:
killer cell immunoglobulin-like receptor, three domains, long cytoplasmic tail, 1
Transcript:
RefSeq NM_177749.3 (NON-CURRENT)
Match location:
Position 674 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000420399)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATCAAGATCACAGGTATATA
Hairpin Sequence:
5'-CCGG-CATCAAGATCACAGGTATATA-CTCGAG-TATATACCTGTGATCTTGATG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATCAAGATCACAGGTATATACTCGAGTATATACCTGTGATCTTGATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATCAAGATCACAGGTATATACTCGAGTATATACCTGTGATCTTGATG-3'

Other clones with same target sequence:

(none)