Construct: shRNA TRCN0000420431

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032396.3-826s21c1
DNA Barcode:
AGGAGCCTCTCGCATCCATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Kremen1 (84035)
Gene Description:
kringle containing transmembrane protein 1
Transcript:
RefSeq NM_032396.3 (CURRENT)
Match location:
Position 826 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000420431)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGGAGCCTCTCGCATCCATTT
Hairpin Sequence:
5'-CCGG-AGGAGCCTCTCGCATCCATTT-CTCGAG-AAATGGATGCGAGAGGCTCCT-TTTTTGGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGGAGCCTCTCGCATCCATTTCTCGAGAAATGGATGCGAGAGGCTCCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGGAGCCTCTCGCATCCATTTCTCGAGAAATGGATGCGAGAGGCTCCT-3'

Other clones with same target sequence:

(none)