Construct: shRNA TRCN0000420448

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016581.3-1238s21c1
DNA Barcode:
GACGATGGCTAAGTGGATCCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ECSIT (51295)
Gene Description:
ECSIT signaling integrator
Transcript:
RefSeq NM_016581.3 (NON-CURRENT)
Match location:
Position 1238 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000420448)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACGATGGCTAAGTGGATCCA
Hairpin Sequence:
5'-CCGG-GACGATGGCTAAGTGGATCCA-CTCGAG-TGGATCCACTTAGCCATCGTC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACGATGGCTAAGTGGATCCACTCGAGTGGATCCACTTAGCCATCGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACGATGGCTAAGTGGATCCACTCGAGTGGATCCACTTAGCCATCGTC-3'

Other clones with same target sequence:

(none)