Construct: shRNA TRCN0000420453

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133853.3-3635s21c1
DNA Barcode:
TGGTGACCAGATCGTTGAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Magi3 (99470)
Gene Description:
membrane associated guanylate kinase, WW and PDZ domain containing 3
Transcript:
RefSeq NM_133853.3 (CURRENT)
Match location:
Position 3635 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000420453)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGGTGACCAGATCGTTGAAAT
Hairpin Sequence:
5'-CCGG-TGGTGACCAGATCGTTGAAAT-CTCGAG-ATTTCAACGATCTGGTCACCA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGGTGACCAGATCGTTGAAATCTCGAGATTTCAACGATCTGGTCACCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGGTGACCAGATCGTTGAAATCTCGAGATTTCAACGATCTGGTCACCA-3'

Other clones with same target sequence:

(none)