Construct: shRNA TRCN0000420479

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020927.1-354s21c1
DNA Barcode:
TGGTGCGACAAGGGAATATTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
VAT1L (57687)
Gene Description:
vesicle amine transport 1 like
Transcript:
RefSeq NM_020927.1 (NON-CURRENT)
Match location:
Position 354 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000420479)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGGTGCGACAAGGGAATATTG
Hairpin Sequence:
5'-CCGG-TGGTGCGACAAGGGAATATTG-CTCGAG-CAATATTCCCTTGTCGCACCA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGGTGCGACAAGGGAATATTGCTCGAGCAATATTCCCTTGTCGCACCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGGTGCGACAAGGGAATATTGCTCGAGCAATATTCCCTTGTCGCACCA-3'

Other clones with same target sequence:

(none)