Construct: shRNA TRCN0000420528

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015507.3-1944s21c1
DNA Barcode:
GACTGAATGTTACTATCTTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EGFL6 (25975)
Gene Description:
EGF like domain multiple 6
Transcript:
RefSeq NM_015507.3 (NON-CURRENT)
Match location:
Position 1944 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000420528)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACTGAATGTTACTATCTTTA
Hairpin Sequence:
5'-CCGG-GACTGAATGTTACTATCTTTA-CTCGAG-TAAAGATAGTAACATTCAGTC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACTGAATGTTACTATCTTTACTCGAGTAAAGATAGTAACATTCAGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACTGAATGTTACTATCTTTACTCGAGTAAAGATAGTAACATTCAGTC-3'

Other clones with same target sequence:

(none)