Construct: shRNA TRCN0000420563

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173561.1-1010s21c1
DNA Barcode:
GTGCCTGAAGCTCACGTACAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
UNC5CL (222643)
Gene Description:
unc-5 family C-terminal like
Transcript:
RefSeq NM_173561.1 (NON-CURRENT)
Match location:
Position 1010 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000420563)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGCCTGAAGCTCACGTACAT
Hairpin Sequence:
5'-CCGG-GTGCCTGAAGCTCACGTACAT-CTCGAG-ATGTACGTGAGCTTCAGGCAC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGCCTGAAGCTCACGTACATCTCGAGATGTACGTGAGCTTCAGGCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGCCTGAAGCTCACGTACATCTCGAGATGTACGTGAGCTTCAGGCAC-3'

Other clones with same target sequence:

(none)