Construct: shRNA TRCN0000420576

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017519.1-6878s21c1
DNA Barcode:
GAAGATTAGAGGGTCACATAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ARID1B (57492)
Gene Description:
AT-rich interaction domain 1B
Transcript:
RefSeq NM_017519.1 (NON-CURRENT)
Match location:
Position 6878 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000420576)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGATTAGAGGGTCACATAT
Hairpin Sequence:
5'-CCGG-GAAGATTAGAGGGTCACATAT-CTCGAG-ATATGTGACCCTCTAATCTTC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGATTAGAGGGTCACATATCTCGAGATATGTGACCCTCTAATCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGATTAGAGGGTCACATATCTCGAGATATGTGACCCTCTAATCTTC-3'

Other clones with same target sequence:

(none)