Construct: shRNA TRCN0000420593

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000980.2-139s21c1
DNA Barcode:
CTAATCATGTCGTCGCCAAGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RPL18A (6142)
Gene Description:
ribosomal protein L18a
Transcript:
RefSeq NM_000980.2 (NON-CURRENT)
Match location:
Position 139 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000420593)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTAATCATGTCGTCGCCAAGT
Hairpin Sequence:
5'-CCGG-CTAATCATGTCGTCGCCAAGT-CTCGAG-ACTTGGCGACGACATGATTAG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTAATCATGTCGTCGCCAAGTCTCGAGACTTGGCGACGACATGATTAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTAATCATGTCGTCGCCAAGTCTCGAGACTTGGCGACGACATGATTAG-3'

Other clones with same target sequence:

(none)