Construct: shRNA TRCN0000420594

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_138424.1-671s21c1
DNA Barcode:
GAGCTGATGCTTGAGGCTAAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KIF12 (113220)
Gene Description:
kinesin family member 12
Transcript:
RefSeq NM_138424.1 (CURRENT)
Match location:
Position 671 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000420594)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGCTGATGCTTGAGGCTAAC
Hairpin Sequence:
5'-CCGG-GAGCTGATGCTTGAGGCTAAC-CTCGAG-GTTAGCCTCAAGCATCAGCTC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGCTGATGCTTGAGGCTAACCTCGAGGTTAGCCTCAAGCATCAGCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGCTGATGCTTGAGGCTAACCTCGAGGTTAGCCTCAAGCATCAGCTC-3'

Other clones with same target sequence:

(none)