Construct: shRNA TRCN0000420620

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133465.2-1455s21c1
DNA Barcode:
CCTTGAATGTGTGGCGTTATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KIAA1958 (158405)
Gene Description:
KIAA1958
Transcript:
RefSeq NM_133465.2 (NON-CURRENT)
Match location:
Position 1455 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000420620)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTGAATGTGTGGCGTTATT
Hairpin Sequence:
5'-CCCG-CCTTGAATGTGTGGCGTTATT-CTCGAG-AATAACGCCACACATTCAAGG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTGAATGTGTGGCGTTATTCTCGAGAATAACGCCACACATTCAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTGAATGTGTGGCGTTATTCTCGAGAATAACGCCACACATTCAAGG-3'

Other clones with same target sequence:

(none)