Construct: shRNA TRCN0000420647

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001542.2-3734s21c1
DNA Barcode:
GGTATTCCCTGAGGACTAAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
IGSF3 (3321)
Gene Description:
immunoglobulin superfamily member 3
Transcript:
RefSeq NM_001542.2 (NON-CURRENT)
Match location:
Position 3734 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000420647)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGTATTCCCTGAGGACTAAAG
Hairpin Sequence:
5'-CCGG-GGTATTCCCTGAGGACTAAAG-CTCGAG-CTTTAGTCCTCAGGGAATACC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGTATTCCCTGAGGACTAAAGCTCGAGCTTTAGTCCTCAGGGAATACCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGTATTCCCTGAGGACTAAAGCTCGAGCTTTAGTCCTCAGGGAATACC-3'

Other clones with same target sequence:

(none)