Construct: shRNA TRCN0000420691

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015321.2-648s21c1
DNA Barcode:
GAATGGAAGAGACCACATCAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CRTC1 (23373)
Gene Description:
CREB regulated transcription coactivator 1
Transcript:
RefSeq NM_015321.2 (NON-CURRENT)
Match location:
Position 648 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000420691)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAATGGAAGAGACCACATCAG
Hairpin Sequence:
5'-CCGG-GAATGGAAGAGACCACATCAG-CTCGAG-CTGATGTGGTCTCTTCCATTC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAATGGAAGAGACCACATCAGCTCGAGCTGATGTGGTCTCTTCCATTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAATGGAAGAGACCACATCAGCTCGAGCTGATGTGGTCTCTTCCATTC-3'

Other clones with same target sequence:

(none)