Construct: shRNA TRCN0000420728

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021311.3-2319s21c1
DNA Barcode:
TGAAGACCCTGGTCAATTATG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Piwil1 (57749)
Gene Description:
piwi-like RNA-mediated gene silencing 1
Transcript:
RefSeq NM_021311.3 (CURRENT)
Match location:
Position 2319 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000420728)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGAAGACCCTGGTCAATTATG
Hairpin Sequence:
5'-CCGG-TGAAGACCCTGGTCAATTATG-CTCGAG-CATAATTGACCAGGGTCTTCA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGAAGACCCTGGTCAATTATGCTCGAGCATAATTGACCAGGGTCTTCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGAAGACCCTGGTCAATTATGCTCGAGCATAATTGACCAGGGTCTTCA-3'

Other clones with same target sequence:

(none)