Construct: shRNA TRCN0000420791

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022574.4-2993s21c1
DNA Barcode:
CTTTGGGACATACCAATTAAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GIGYF1 (64599)
Gene Description:
GRB10 interacting GYF protein 1
Transcript:
RefSeq NM_022574.4 (NON-CURRENT)
Match location:
Position 2993 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000420791)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTTGGGACATACCAATTAAC
Hairpin Sequence:
5'-CCGG-CTTTGGGACATACCAATTAAC-CTCGAG-GTTAATTGGTATGTCCCAAAG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTTGGGACATACCAATTAACCTCGAGGTTAATTGGTATGTCCCAAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTTGGGACATACCAATTAACCTCGAGGTTAATTGGTATGTCCCAAAG-3'

Other clones with same target sequence:

(none)