Construct: shRNA TRCN0000420798

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152426.3-1104s21c1
DNA Barcode:
TTCACCATGGAAGTTACAAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
APOBEC3D (140564)
Gene Description:
apolipoprotein B mRNA editing enzyme catalytic subunit 3D
Transcript:
RefSeq NM_152426.3 (NON-CURRENT)
Match location:
Position 1104 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000420798)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTCACCATGGAAGTTACAAAG
Hairpin Sequence:
5'-CCGG-TTCACCATGGAAGTTACAAAG-CTCGAG-CTTTGTAACTTCCATGGTGAA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTCACCATGGAAGTTACAAAGCTCGAGCTTTGTAACTTCCATGGTGAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTCACCATGGAAGTTACAAAGCTCGAGCTTTGTAACTTCCATGGTGAA-3'

Other clones with same target sequence:

(none)