Construct: shRNA TRCN0000420817

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022900.4-1744s21c1
DNA Barcode:
TAGTAATGGATCGACCTTATC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CASD1 (64921)
Gene Description:
CAS1 domain containing 1
Transcript:
RefSeq NM_022900.4 (NON-CURRENT)
Match location:
Position 1744 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000420817)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TAGTAATGGATCGACCTTATC
Hairpin Sequence:
5'-CCGG-TAGTAATGGATCGACCTTATC-CTCGAG-GATAAGGTCGATCCATTACTA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTAGTAATGGATCGACCTTATCCTCGAGGATAAGGTCGATCCATTACTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATAGTAATGGATCGACCTTATCCTCGAGGATAAGGTCGATCCATTACTA-3'

Other clones with same target sequence:

(none)