Construct: shRNA TRCN0000420828

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001563.2-292s21c1
DNA Barcode:
GTCAACTATGAGACGAATATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
IMPG1 (3617)
Gene Description:
interphotoreceptor matrix proteoglycan 1
Transcript:
RefSeq NM_001563.2 (NON-CURRENT)
Match location:
Position 292 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000420828)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCAACTATGAGACGAATATT
Hairpin Sequence:
5'-CCGG-GTCAACTATGAGACGAATATT-CTCGAG-AATATTCGTCTCATAGTTGAC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCAACTATGAGACGAATATTCTCGAGAATATTCGTCTCATAGTTGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCAACTATGAGACGAATATTCTCGAGAATATTCGTCTCATAGTTGAC-3'

Other clones with same target sequence:

(none)