Construct: shRNA TRCN0000420860

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_146102.1-2684s21c1
DNA Barcode:
GCAAACGGAGCTTCGGTTAAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Afap1l2 (226250)
Gene Description:
actin filament associated protein 1-like 2
Transcript:
RefSeq NM_146102.1 (NON-CURRENT)
Match location:
Position 2684 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000420860)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAAACGGAGCTTCGGTTAAG
Hairpin Sequence:
5'-CCGG-GCAAACGGAGCTTCGGTTAAG-CTCGAG-CTTAACCGAAGCTCCGTTTGC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAAACGGAGCTTCGGTTAAGCTCGAGCTTAACCGAAGCTCCGTTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAAACGGAGCTTCGGTTAAGCTCGAGCTTAACCGAAGCTCCGTTTGC-3'

Other clones with same target sequence:

(none)