Construct: shRNA TRCN0000420887

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178606.4-378s21c1
DNA Barcode:
CTATGAGACAATGGTGAATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Reep3 (28193)
Gene Description:
receptor accessory protein 3
Transcript:
RefSeq NM_178606.4 (NON-CURRENT)
Match location:
Position 378 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000420887)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTATGAGACAATGGTGAATTT
Hairpin Sequence:
5'-CCGG-CTATGAGACAATGGTGAATTT-CTCGAG-AAATTCACCATTGTCTCATAG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTATGAGACAATGGTGAATTTCTCGAGAAATTCACCATTGTCTCATAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTATGAGACAATGGTGAATTTCTCGAGAAATTCACCATTGTCTCATAG-3'

Other clones with same target sequence:

(none)