Construct: shRNA TRCN0000420891

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002374.3-2936s21c1
DNA Barcode:
TTACGAAGGCACTGATGATAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAP2 (4133)
Gene Description:
microtubule associated protein 2
Transcript:
RefSeq NM_002374.3 (CURRENT)
Match location:
Position 2936 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000420891)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTACGAAGGCACTGATGATAA
Hairpin Sequence:
5'-CCGG-TTACGAAGGCACTGATGATAA-CTCGAG-TTATCATCAGTGCCTTCGTAA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTACGAAGGCACTGATGATAACTCGAGTTATCATCAGTGCCTTCGTAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTACGAAGGCACTGATGATAACTCGAGTTATCATCAGTGCCTTCGTAA-3'

Other clones with same target sequence:

(none)