Construct: shRNA TRCN0000420925

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024885.3-1026s21c1
DNA Barcode:
GATGATGAGGATGAGGATGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TAF7L (54457)
Gene Description:
TATA-box binding protein associated factor 7 like
Transcript:
RefSeq NM_024885.3 (NON-CURRENT)
Match location:
Position 1026 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000420925)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATGATGAGGATGAGGATGAT
Hairpin Sequence:
5'-CCGG-GATGATGAGGATGAGGATGAT-CTCGAG-ATCATCCTCATCCTCATCATC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATGATGAGGATGAGGATGATCTCGAGATCATCCTCATCCTCATCATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATGATGAGGATGAGGATGATCTCGAGATCATCCTCATCCTCATCATC-3'

Other clones with same target sequence:

(none)