Construct: shRNA TRCN0000420976

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173822.3-383s21c1
DNA Barcode:
TAGCTCAGAGGTTCGACTTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FAM126B (285172)
Gene Description:
family with sequence similarity 126 member B
Transcript:
RefSeq NM_173822.3 (NON-CURRENT)
Match location:
Position 383 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000420976)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TAGCTCAGAGGTTCGACTTAA
Hairpin Sequence:
5'-CCGG-TAGCTCAGAGGTTCGACTTAA-CTCGAG-TTAAGTCGAACCTCTGAGCTA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTAGCTCAGAGGTTCGACTTAACTCGAGTTAAGTCGAACCTCTGAGCTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATAGCTCAGAGGTTCGACTTAACTCGAGTTAAGTCGAACCTCTGAGCTA-3'

Other clones with same target sequence:

(none)