Construct: shRNA TRCN0000420991
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_177712.4-772s21c1
- DNA Barcode:
- AGTGTGAAGAGTGTGGAAATT
Original Target:
- Taxon:
- Mus musculus (mouse)
- Gene:
- Zfp874a (238692)
- Gene Description:
- zinc finger protein 874a
- Transcript:
- RefSeq NM_177712.4 (CURRENT)
- Match location:
- Position 772 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000420991)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
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Sequence Information
- Target Sequence:
- AGTGTGAAGAGTGTGGAAATT
- Hairpin Sequence:
- 5'-CCGG-AGTGTGAAGAGTGTGGAAATT-CTCGAG-AATTTCCACACTCTTCACACT-TTTTTTGAAT-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGAGTGTGAAGAGTGTGGAAATTCTCGAGAATTTCCACACTCTTCACACTTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAAGTGTGAAGAGTGTGGAAATTCTCGAGAATTTCCACACTCTTCACACT-3'