Construct: shRNA TRCN0000420993

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008604.3-1197s21c1
DNA Barcode:
TAATGTCCTGGAGGTTCATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mme (17380)
Gene Description:
membrane metallo endopeptidase
Transcript:
RefSeq NM_008604.3 (NON-CURRENT)
Match location:
Position 1197 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000420993)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TAATGTCCTGGAGGTTCATAA
Hairpin Sequence:
5'-CCGG-TAATGTCCTGGAGGTTCATAA-CTCGAG-TTATGAACCTCCAGGACATTA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTAATGTCCTGGAGGTTCATAACTCGAGTTATGAACCTCCAGGACATTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATAATGTCCTGGAGGTTCATAACTCGAGTTATGAACCTCCAGGACATTA-3'

Other clones with same target sequence:

(none)