Construct: shRNA TRCN0000421039

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145284.5-923s21c1
DNA Barcode:
TAACTGCTGTTTCCTACATAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FAM122B (159090)
Gene Description:
family with sequence similarity 122B
Transcript:
RefSeq NM_145284.5 (CURRENT)
Match location:
Position 923 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000421039)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TAACTGCTGTTTCCTACATAA
Hairpin Sequence:
5'-CCGG-TAACTGCTGTTTCCTACATAA-CTCGAG-TTATGTAGGAAACAGCAGTTA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTAACTGCTGTTTCCTACATAACTCGAGTTATGTAGGAAACAGCAGTTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATAACTGCTGTTTCCTACATAACTCGAGTTATGTAGGAAACAGCAGTTA-3'

Other clones with same target sequence:

(none)