Construct: shRNA TRCN0000421040

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021254.1-408s21c1
DNA Barcode:
AGGAATGGGCAAGCTCCAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CFAP298 (56683)
Gene Description:
cilia and flagella associated protein 298
Transcript:
RefSeq NM_021254.1 (NON-CURRENT)
Match location:
Position 408 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000421040)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGGAATGGGCAAGCTCCAAAT
Hairpin Sequence:
5'-CCGG-AGGAATGGGCAAGCTCCAAAT-CTCGAG-ATTTGGAGCTTGCCCATTCCT-TTTTTTTTAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGGAATGGGCAAGCTCCAAATCTCGAGATTTGGAGCTTGCCCATTCCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGGAATGGGCAAGCTCCAAATCTCGAGATTTGGAGCTTGCCCATTCCT-3'

Other clones with same target sequence:

(none)