Construct: shRNA TRCN0000421046

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007153.3-1921s21c1
DNA Barcode:
ACCAATCTGCAATTCTTATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZNF208 (7757)
Gene Description:
zinc finger protein 208
Transcript:
RefSeq NM_007153.3 (CURRENT)
Match location:
Position 1921 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000421046)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACCAATCTGCAATTCTTATTA
Hairpin Sequence:
5'-CCGG-ACCAATCTGCAATTCTTATTA-CTCGAG-TAATAAGAATTGCAGATTGGT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACCAATCTGCAATTCTTATTACTCGAGTAATAAGAATTGCAGATTGGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACCAATCTGCAATTCTTATTACTCGAGTAATAAGAATTGCAGATTGGT-3'

Other clones with same target sequence:

(none)