Construct: shRNA TRCN0000421057
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_011489.3-2212s21c1
- DNA Barcode:
- GAATTTGCCAGGACGGAATTA
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Stat5b (20851)
- Gene Description:
- signal transducer and activator of transcription 5B
- Transcript:
- RefSeq NM_011489.3 (CURRENT)
- Match location:
- Position 2212 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000421057)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GAATTTGCCAGGACGGAATTA
- Hairpin Sequence:
-
5'-CCGG-GAATTTGCCAGGACGGAATTA-CTCGAG-TAATTCCGTCCTGGCAAATTC-TTTTTTGAAT-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGAATTTGCCAGGACGGAATTACTCGAGTAATTCCGTCCTGGCAAATTCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGAATTTGCCAGGACGGAATTACTCGAGTAATTCCGTCCTGGCAAATTC-3'
Other clones with same target sequence:
(none)
