Construct: shRNA TRCN0000421072

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_033178.2-491s21c1
DNA Barcode:
AGATTTGGTTTCAGAATGAGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DUX4L1 (22947)
Gene Description:
double homeobox 4 like 1 (pseudogene)
Transcript:
RefSeq NM_033178.2 (NON-CURRENT)
Match location:
Position 491 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000421072)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGATTTGGTTTCAGAATGAGA
Hairpin Sequence:
5'-CCGG-AGATTTGGTTTCAGAATGAGA-CTCGAG-TCTCATTCTGAAACCAAATCT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGATTTGGTTTCAGAATGAGACTCGAGTCTCATTCTGAAACCAAATCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGATTTGGTTTCAGAATGAGACTCGAGTCTCATTCTGAAACCAAATCT-3'

Other clones with same target sequence:

(none)