Construct: shRNA TRCN0000421142

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022458.3-741s21c1
DNA Barcode:
GCCGCAAGCATGGAATCTTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LMBR1 (64327)
Gene Description:
limb development membrane protein 1
Transcript:
RefSeq NM_022458.3 (NON-CURRENT)
Match location:
Position 741 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000421142)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCGCAAGCATGGAATCTTTA
Hairpin Sequence:
5'-CCGG-GCCGCAAGCATGGAATCTTTA-CTCGAG-TAAAGATTCCATGCTTGCGGC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCGCAAGCATGGAATCTTTACTCGAGTAAAGATTCCATGCTTGCGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCGCAAGCATGGAATCTTTACTCGAGTAAAGATTCCATGCTTGCGGC-3'

Other clones with same target sequence:

(none)