Construct: shRNA TRCN0000421222

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145267.2-96s21c1
DNA Barcode:
CTGTGTCATTCTCTGAGGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SDHAF4 (135154)
Gene Description:
succinate dehydrogenase complex assembly factor 4
Transcript:
RefSeq NM_145267.2 (NON-CURRENT)
Match location:
Position 96 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000421222)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGTGTCATTCTCTGAGGAAA
Hairpin Sequence:
5'-CCGG-CTGTGTCATTCTCTGAGGAAA-CTCGAG-TTTCCTCAGAGAATGACACAG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGTGTCATTCTCTGAGGAAACTCGAGTTTCCTCAGAGAATGACACAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGTGTCATTCTCTGAGGAAACTCGAGTTTCCTCAGAGAATGACACAG-3'

Other clones with same target sequence:

(none)