Construct: shRNA TRCN0000421224

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015320.2-1375s21c1
DNA Barcode:
GACCAACGTCATCAACGAGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ARHGEF4 (50649)
Gene Description:
Rho guanine nucleotide exchange factor 4
Transcript:
RefSeq NM_015320.2 (NON-CURRENT)
Match location:
Position 1375 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000421224)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACCAACGTCATCAACGAGAT
Hairpin Sequence:
5'-CCGG-GACCAACGTCATCAACGAGAT-CTCGAG-ATCTCGTTGATGACGTTGGTC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACCAACGTCATCAACGAGATCTCGAGATCTCGTTGATGACGTTGGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACCAACGTCATCAACGAGATCTCGAGATCTCGTTGATGACGTTGGTC-3'

Other clones with same target sequence:

(none)