Construct: shRNA TRCN0000421225

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153000.4-2156s21c1
DNA Barcode:
AGTTCTGGACGAGCGTGTTTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
APCDD1 (147495)
Gene Description:
APC down-regulated 1
Transcript:
RefSeq NM_153000.4 (NON-CURRENT)
Match location:
Position 2156 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000421225)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGTTCTGGACGAGCGTGTTTG
Hairpin Sequence:
5'-CCGG-AGTTCTGGACGAGCGTGTTTG-CTCGAG-CAAACACGCTCGTCCAGAACT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGTTCTGGACGAGCGTGTTTGCTCGAGCAAACACGCTCGTCCAGAACTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGTTCTGGACGAGCGTGTTTGCTCGAGCAAACACGCTCGTCCAGAACT-3'

Other clones with same target sequence:

(none)